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Cyanobacteria mediated green synthesis of gold-silver nanoalloy

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Abstract

Green synthesis is a significant process for non-toxic metal nanoparticle production employing plant materials. In the present investigation we reported an environmentally friendly method for gold-silver nano-alloy biosynthesis using a cyanobacterial strain, Lyngbya majuscula as bio-reagent. On exposure of Lyngbya thallus to equimolar solution of gold and silver (1 mM, pH 4) for 72 hrs and subsequent pink coloration of the experimental biomass indicated the nanoparticle synthesis. Extracted nanoparticles showed a distinct single plasmon band at 481 nm and further EDX analysis confirmed the presence of both the metals Au and Ag in nano-alloy form. It was also observed from TEM study that all the synthesized particles were spherical in nature with a size range of ~5-25 nm. The SEM pictures of the metal treated biomass confirmed the intra and extracellular nano-alloy formation. The XRD analysis of particle loaded biomass revealed that the 2θ values appeared at 38.2°, 44.5°, 64.8°and 77.8° that were indexed at (111), (200), (220) and (311) lattice planes. Presence of different functional groups viz. N-H, C=C, C–O, C=O on the surface of nanoparticles were recorded by FTIR. Overall, this technique requires low energy and less manufacturing cost for metal alloy biosynthesis.

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Abbreviations

Au-Ag NPs:

Gold-silver alloy nanoparticles

TEM:

Transmission electron microscopy

SEM:

Scanning electron microscopy

EDX:

Energy dispersive X-ray

FTIR:

Fourier transform infrared spectroscopy

XRD:

X-ray diffraction

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Acknowledgments

Authors would like to thank University Grants Commission (UGC) for financial support to first author as

UGC-Adhoc Fellowship and Urmila Goswami, Tridib Das and Prasun Mukherjee for their immense help during this research work.

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Authors and Affiliations

  1. Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    Piya Roychoudhury, Satabdi Ghosh & Ruma Pal

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  1. Piya Roychoudhury
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  2. Satabdi Ghosh
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  3. Ruma Pal
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Correspondence to Ruma Pal.

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Roychoudhury, P., Ghosh, S. & Pal, R. Cyanobacteria mediated green synthesis of gold-silver nanoalloy. J. Plant Biochem. Biotechnol. 25, 73–78 (2016). https://doi.org/10.1007/s13562-015-0311-0

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  • DOI: https://doi.org/10.1007/s13562-015-0311-0

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